一、光學顯微鏡的分類方法
光(guang)(guang)學顯(xian)(xian)微(wei)(wei)(wei)(wei)鏡有(you)多種(zhong)分(fen)(fen)(fen)類(lei)方法,按(an)(an)使用目鏡的(de)數目可(ke)分(fen)(fen)(fen)為(wei)三目,雙目和(he)單目顯(xian)(xian)微(wei)(wei)(wei)(wei)鏡;按(an)(an)圖(tu)像是否有(you)立(li)體感可(ke)分(fen)(fen)(fen)為(wei)立(li)體視(shi)覺(jue)和(he)非(fei)立(li)體視(shi)覺(jue)顯(xian)(xian)微(wei)(wei)(wei)(wei)鏡;按(an)(an)觀察對像可(ke)分(fen)(fen)(fen)為(wei)生物和(he)金(jin)相(xiang)顯(xian)(xian)微(wei)(wei)(wei)(wei)鏡等;按(an)(an)光(guang)(guang)學原理(li)可(ke)分(fen)(fen)(fen)為(wei)偏光(guang)(guang),相(xiang)襯和(he)微(wei)(wei)(wei)(wei)分(fen)(fen)(fen)干涉對比顯(xian)(xian)微(wei)(wei)(wei)(wei)鏡等;按(an)(an)光(guang)(guang)源類(lei)型(xing)可(ke)分(fen)(fen)(fen)為(wei)普通(tong)光(guang)(guang)、熒(ying)光(guang)(guang)、紅外光(guang)(guang)和(he)激光(guang)(guang)顯(xian)(xian)微(wei)(wei)(wei)(wei)鏡等;按(an)(an)接收器(qi)類(lei)型(xing)可(ke)分(fen)(fen)(fen)為(wei)目視(shi)、攝(she)影和(he)電(dian)視(shi)顯(xian)(xian)微(wei)(wei)(wei)(wei)鏡等。常(chang)用的(de)顯(xian)(xian)微(wei)(wei)(wei)(wei)鏡有(you)雙目連續(xu)變(bian)倍體視(shi)顯(xian)(xian)微(wei)(wei)(wei)(wei)鏡、金(jin)相(xiang)顯(xian)(xian)微(wei)(wei)(wei)(wei)鏡、偏光(guang)(guang)顯(xian)(xian)微(wei)(wei)(wei)(wei)鏡、紫(zi)外熒(ying)光(guang)(guang)顯(xian)(xian)微(wei)(wei)(wei)(wei)鏡等。
二、光學顯微鏡的使用規程
1、實驗時要把顯微鏡放在桌(zhuo)面上稍偏(pian)左的位置,鏡座應距桌(zhuo)沿6~7 cm左右。
2、打開光源(yuan)開關,調節光強(qiang)到合適大(da)小。
3、轉動物(wu)鏡(jing)轉換器(qi),使低(di)倍鏡(jing)頭正對載(zai)物(wu)臺上的通光(guang)(guang)孔。先把鏡(jing)頭調節(jie)至(zhi)距(ju)載(zai)物(wu)臺1~2cm左(zuo)(zuo)右處,然后用(yong)左(zuo)(zuo)眼注視目鏡(jing)內(nei),接著(zhu)調節(jie)聚光(guang)(guang)器(qi)的高度,把孔徑光(guang)(guang)闌調至(zhi)最大,使光(guang)(guang)線通過聚光(guang)(guang)器(qi)射(she)入到鏡(jing)筒內(nei),這時視野內(nei)呈明(ming)亮的狀(zhuang)態。
4、將所(suo)要觀(guan)察的(de)玻(bo)片(pian)放(fang)在載(zai)(zai)物臺上,使玻(bo)片(pian)中被觀(guan)察的(de)部分位于通(tong)光孔的(de)正中央,然后用標本夾(jia)夾(jia)好載(zai)(zai)玻(bo)片(pian)。
5、先用低倍鏡(jing)(jing)觀察(cha)(物(wu)(wu)鏡(jing)(jing)10X、目鏡(jing)(jing)10x)。觀察(cha)之前,先轉動粗動調(diao)焦手輪(lun),使載物(wu)(wu)臺上升,物(wu)(wu)鏡(jing)(jing)逐漸(jian)接近玻(bo)片。需要注意,不能(neng)使物(wu)(wu)鏡(jing)(jing)觸及(ji)玻(bo)片,以防鏡(jing)(jing)頭(tou)將玻(bo)片壓(ya)碎。然(ran)后,左(zuo)眼(yan)注視(shi)目鏡(jing)(jing)內,同(tong)時右(you)眼(yan)不要閉合(he)(要養成睜開雙眼(yan)用顯微鏡(jing)(jing)進(jin)行(xing)觀察(cha)的習慣(guan),以便(bian)在觀察(cha)的同(tong)時能(neng)用右(you)眼(yan)看(kan)著繪圖),并轉動粗動調(diao)焦手輪(lun),使載物(wu)(wu)臺慢(man)慢(man)下降(jiang),不久即可看(kan)到玻(bo)片中材料的放大物(wu)(wu)像。
6、如(ru)果(guo)在視(shi)野(ye)內看到的物(wu)(wu)像(xiang)(xiang)(xiang)不(bu)符合實驗要求(物(wu)(wu)像(xiang)(xiang)(xiang)偏離視(shi)野(ye)),可慢(man)慢(man)調(diao)節(jie)載物(wu)(wu)臺移(yi)動(dong)手柄。調(diao)節(jie)時應注意,玻片移(yi)動(dong)的方(fang)向(xiang)與視(shi)野(ye)中看到的物(wu)(wu)像(xiang)(xiang)(xiang)移(yi)動(dong)的方(fang)向(xiang),正好(hao)相反(fan)。如(ru)果(guo)物(wu)(wu)像(xiang)(xiang)(xiang)不(bu)甚清(qing)晰,可以調(diao)節(jie)微動(dong)調(diao)焦手輪,直至物(wu)(wu)像(xiang)(xiang)(xiang)清(qing)晰為止。
7、一般(ban)具有正常功(gong)能(neng)的顯微(wei)鏡(jing),低(di)(di)倍物(wu)(wu)鏡(jing)和高倍物(wu)(wu)鏡(jing)基本齊焦,在用低(di)(di)倍物(wu)(wu)鏡(jing)觀察清晰時,換高倍物(wu)(wu)鏡(jing)應可以見到物(wu)(wu)像,但物(wu)(wu)像不(bu)一定很清晰,可以轉動微(wei)動調焦手輪進行(xing)調節。
8、在轉換高倍物鏡(jing)并且(qie)看清物像之后,可(ke)以(yi)根(gen)據(ju)需要調節孔徑光(guang)闌的大小(xiao)或聚光(guang)器的高低,使光(guang)線符合要求(qiu)(一般將低倍物鏡(jing)換成(cheng)高倍物鏡(jing)觀(guan)察(cha)時,視野要稍變暗一些,所(suo)以(yi)需要調節光(guang)線強弱(ruo))。
9、觀察完(wan)(wan)畢(bi),應先(xian)將物(wu)鏡(jing)(jing)鏡(jing)(jing)頭(tou)從通光孔處移開(kai),然后將孔徑光闌調至最大(da),再將載物(wu)臺緩(huan)緩(huan)落下,并檢(jian)查零件(jian)有無損傷(特別要注意(yi)檢(jian)查物(wu)鏡(jing)(jing)是否沾水沾油(you),如沾了水或油(you)要用鏡(jing)(jing)頭(tou)紙擦凈(jing)),檢(jian)查處理完(wan)(wan)畢(bi)后即可裝箱。